Forensic application of reverse dot blot with biotin incorporation.
- Author:
Wei Bin JIANG
1
;
Xian Hua JIANG
Author Information
1. Forensic Science Institute of Liaoning Province, Shenyang 110032.
- Publication Type:Journal Article
- MeSH:
Alleles;
Biotin;
DNA/analysis*;
Forensic Medicine/methods*;
Gene Frequency;
Genotype;
HLA-DQ Antigens/genetics*;
HLA-DQ alpha-Chains;
Humans;
Polymerase Chain Reaction/methods*
- From:
Journal of Forensic Medicine
2001;17(1):12-61
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and accurate reverse dot blot technique was successfully established. It could distinguish the 6 alleles of 0101, 0102, 0103, 0201, 0301 and 0401 of HLA-DQA locus. Alleles and genotype frequencies for HLA-DQA locus were determined in 200 unrelated individuals of northern Han population. The discrimination power (DP) was 0.928, heterozygocity (H) 0.75. By direct biotin incorperation, 1 ng genome DNA could be detected successfully. This method can be used for paternity test and individual identification in forensic science practice.
