In vivo distribution and expression after skin injury of fibronectin splicing variants.
- Author:
Yi Jiu CHEN
1
;
Ning Guo LIU
;
Xiao Hua HUANG
;
Li LI
Author Information
1. Institute of Forensic Science, Ministry of Justice, Shanghai 200063. yijiuchen@yahoo.com.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Animals, Newborn;
Fibronectins/metabolism*;
RNA Splicing;
Rats;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction;
Skin/metabolism*
- From:
Journal of Forensic Medicine
2001;17(4):193-195
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:In order to develop new criteria and to select positive controls for forensic medicine, experiments on in vivo distribution and expression after skin wound of fibronectin splicing variants, EIIIA and EIIIB were studied.
METHODS:Rat tissues were homogenized to extract total RNA which was then amplified by RT-PCR technique.
RESULTS:(1) EIIIA+ and EIIIB+ of normal skin were not expressed, whereas EIIIA+ after 18 hours' injury was expressed abundantly. No obvious charge of EIIIB+ was detected. The differences between results of using two methods of injury were not significant. (2) The distribution of fibronectin variants in different adult organs were in the following order: brain > heart > liver.
CONCLUSIONS:EIIIA+ may be used as a sensitive criterion for research of skin injury.
