Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application.
- Author:
Yue LI
1
;
Sui Bao WANG
;
Chao LIU
;
Hong Xia LI
;
Hui Ying HU
;
Hong LIU
;
Chang hui LIU
;
Xiao Hui CHEN
Author Information
1. Guangzhou Criminal Science & Technology Institute, Guangzhou 510030.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Asian People/genetics*;
China;
Female;
Gene Frequency;
Genetics, Population;
Humans;
Male;
Paternity;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2001;17(2):82-85
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The genetic studies of 13 short tandem repeats(STRs) loci in two multiplex amplification systems were carried out on Chinese Han population in Guangdong.
METHODS:DNA samples from 328 unrelated individuals were screened. The 13 loci were D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, TH01, TPOX and CSF1PO. The PCR products were analyzed and genotyped by ABI 377-96 Sequencer.
RESULTS:The combined power of discrimination (DP) was 0.999999999999993 and the combined paternity of exclusion(PE) was 99.999%. These 13 loci met the Hardy-Weinberg expectations.
CONCLUSION:The two multiplex amplification systems were very useful in forensic case investigation.
