A study on pathological changes of closed diffuse brain injury in rats with H.E., esterification-silver stain and albumin immunohistochemical stain.
- Author:
Xiao jun YU
1
;
Fei XIAO
;
Jia Wen WU
;
Mei Yun WU
Author Information
1. Department of Forensic Medicine, Medical College of Beihua University, Jilin 132001.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Albumins;
Animals;
Biomechanical Phenomena;
Brain/pathology*;
Head Injuries, Closed/pathology*;
Immunohistochemistry/methods*;
Male;
Rats;
Rats, Sprague-Dawley;
Silver Staining
- From:
Journal of Forensic Medicine
2001;17(2):69-76
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observed the pathological changes of closed diffuse brain injury in the rats died immediately and 15 min to 5 days after the injury.
METHODS:H.E. staind and esterification-silver stain were applied to investigate the closed diffuse brain injury.
RESULTS:In rats died immediately after the concussive injury, a number of shrunken neurons(type I change), distended neurons(type II change) and wave-like nerve fibers were identified in the brain tissue, especially in brain-stem. At 2 h and 8 h after injury, brain edema and axonal swelling appeared clearly in the cortex and white matter, especially in brain-stem. At post-traumatic 8 h and 24 h, the axonal retraction balls began to appear. The amount of neurons undergoing type I and II changes and constraction balls increased along with the survivor time. After 4 days and 5 days, brain edema alleviated, but the retraction balls and axonal swelling still existed. With Esterifica-tion silver stain, the above changes of neurons and nerve fibers were more obvious. With ABC stain, the distribution of albumin(Al) was extended from the perio-vascular area to diffuse distribution. Al positive staining were more obvious in injuried neurons and nerve fibers.
CONCLUSION:The distribution of the concussive damage in the brain are coup, contra-coup and centripental.
