The mechanism of S100A7 inducing the migration and invasion in cervical cancer cells.
10.3760/cma.j.cn112152-20210804-00576
- VernacularTitle:钙结合蛋白S100A7通过上皮－间质转化促进宫颈癌细胞侵袭迁移
- Author:
Tian TIAN
1
;
Zhen HUA
2
;
Yan KONG
1
;
Ling Zhi WANG
1
;
Xiang Yu LIU
1
;
Yi HAN
1
;
Xue Min ZHOU
3
;
Zhu Mei CUI
1
Author Information
1. Department of Gynecology, the Affiliated Hospital of Qingdao University, Qingdao 266061, China.
2. Department of Anesthesiology, the Affiliated Hospital of Qingdao University, Qingdao 266061, China.
3. Department of Clinical Medicine, Qingdao University, Qingdao 266071, China.
- Publication Type:Journal Article
- Keywords:
Epithelial-mesenchymal transition;
Exosome;
Metastasis;
S100A7;
Uterine cervical neoplasms
- MeSH:
Female;
Humans;
Uterine Cervical Neoplasms/pathology*;
HeLa Cells;
Fibronectins/metabolism*;
Culture Media, Conditioned;
Carcinoma, Squamous Cell/metabolism*;
Adenocarcinoma;
Cadherins/metabolism*;
RNA, Messenger/metabolism*;
Cell Movement;
Epithelial-Mesenchymal Transition/genetics*;
Cell Line, Tumor;
Cell Proliferation;
S100 Calcium Binding Protein A7/metabolism*
- From:
Chinese Journal of Oncology
2023;45(5):375-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of S100A7 inducing the migration and invasion in cervical cancers. Methods: Tissue samples of 5 cases of cervical squamous cell carcinoma and 3 cases of adenocarcinoma were collected from May 2007 to December 2007 in the Department of Gynecology of the Affiliated Hospital of Qingdao University. Immunohistochemistry was performed to evaluate the expression of S100A7 in cervical carcinoma tissues. S100A7-overexpressing HeLa and C33A cells were established with lentiviral systems as the experimental group. Immunofluorescence assay was performed to observe the cell morphology. Transwell assay was taken to detect the effect of S100A7-overexpression on the migration and invasion of cervical cancer cells. Reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) was used to examine the mRNA expressions of E-cadherin, N-cadherin, vimentin and fibronectin. The expression of extracellular S100A7 in conditioned medium of cervical cancer cell was detected by western blot. Conditioned medium was added into Transwell lower compartment to detect cell motility. Exosomes were isolated and extracted from the culture supernatant of cervical cancer cell, the expressions of S100A7, CD81 and TSG101 were detected by western blot. Transwell assay was taken to detect the effect of exosomes on the migration and invasion of cervical cancer cells. Results: S100A7 expression was positively expressed in cervical squamous carcinoma and negative expression in adenocarcinoma. Stable S100A7-overexpressing HeLa and C33A cells were successfully constructed. C33A cells in the experimental group were spindle shaped while those in the control group tended to be polygonal epithelioid cells. The number of S100A7-overexpressed HeLa cells passing through the Transwell membrane assay was increased significantly in migration and invasion assay (152.00±39.22 vs 105.13±15.75, P<0.05; 115.38±34.57 vs 79.50±13.68, P<0.05). RT-qPCR indicated that the mRNA expressions of E-cadherin in S100A7-overexpressed HeLa and C33A cells decreased (P<0.05) while the mRNA expressions of N-cadherin and fibronectin in HeLa cells and fibronectin in C33A cells increased (P<0.05). Western blot showed that extracellular S100A7 was detected in culture supernatant of cervical cancer cells. HeLa cells of the experimental group passing through transwell membrane in migration and invasion assays were increased significantly (192.60±24.41 vs 98.80±47.24, P<0.05; 105.40±27.38 vs 84.50±13.51, P<0.05) when the conditional medium was added into the lower compartment of Transwell. Exosomes from C33A cell culture supernatant were extracted successfully, and S100A7 expression was positive. The number of transmembrane C33A cells incubated with exosomes extracted from cells of the experimental group was increased significantly (251.00±49.82 vs 143.00±30.85, P<0.05; 524.60±52.74 vs 389.00±63.23, P<0.05). Conclusion: S100A7 may promote the migration and invasion of cervical cancer cells by epithelial-mesenchymal transition and exosome secretion.
