Analysis of Chemical Constituents in Puerariae Lobatae Radix Dispensing Granules by UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20230762
- VernacularTitle:基于UPLC-Q-TOF-MS/MS技术分析葛根配方颗粒的化学成分
- Author:
Meiqing CHEN
1
;
Weifeng ZHU
2
;
Yongmei GUAN
2
;
Yulin FENG
1
;
Yanli ZHANG
1
;
Xiucun JING
1
;
Wanqian PENG
1
;
Hui OUYANG
1
;
Qiong LI
1
Author Information
1. National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine（TCM），Jiangxi University of Chinese Medicine，Nanchang 330006，China
2. Key Laboratory of Modern Preparation of TCM，Ministry of Education，Jiangxi University of Chinese Medicine，Nanchang 330004，China
- Publication Type:Journal Article
- Keywords:
Puerariae Lobatae Radix dispensing granules;
chemical composition;
flavonoids;
organic acids;
coumarins;
ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry（UPLC-Q-TOF-MS/MS）;
fragmentation patterns
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(19):176-186
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveA rapid method for identification of chemical constituents in Puerariae Lobatae Radix dispensing granules was established in order to clarify the material basis. MethodThe chemical constituents of Puerariae Lobatae Radix dispensing granules was qualitatively analyzed by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry（UPLC-Q-TOF-MS/MS） under positive and negative ion modes， and the chromatographic conditions were on an ACQUITY UPLC HSS T3 column（2.1 mm×100 mm， 1.8 μm） with 0.1% formic acid aqueous solution（A）-0.1% formic acid acetonitrile solution（B） as mobile phase for gradient elution（0-4 min， 5%-10%B； 4-10 min， 10%-15%B； 10-20 min， 15%-16%B； 20-27 min， 16%-31%B； 27-33 min， 31%-59%B； 33-42 min， 59%-95%B； 42-42.1 min， 95%-5%B； 42.1-45 min， 5%B）， the flow rate was 0.35 mL·min-1， the column temperature was 40 ℃， the injection volume was 5 μL， and electrospray ionization（ESI） was selected. Then these chemical constituents were comprehensively identified based on PeakView 1.2， PubChem， ChemicalBook， ChemSpider， comparative control profiles and literature information. ResultA total of 128 chemical constituents were identified from the dispensing granules， including 60 flavonoids， 26 organic acids， 7 glycosides， 6 coumarins， 3 nucleosides and 26 other compounds. By focusing on the cleavage patterns of flavonoids， organic acids， glycosides， coumarins， nucleosides and other compounds， 12 compounds that have not been reported in Puerariae Lobatae Radix species were identified from the dispensing granules. ConclusionThe established method can systematically and rapidly identify the chemical constituents in Puerariae Lobatae Radix dispensing granules， and cleared it composition is mainly flavonoids and organic acids. Laying a foundation for the study of the material basis， mechanism of action and clinical application of the dispensing granules.
