Evaluation of anti-quorum sensing potential of Averrhoa bilimbi (Kamias) against Pseudomonas aeruginosa ATCC 27853.
- Author:
Mark Gabriel M. Delos Santos
1
;
Joanna J. Orejola
1
Author Information
- Publication Type:Journal Article
- Keywords: kamias; swarming motility
- MeSH: Pseudomonas aeruginosa; Averrhoa bilimbi; quorum sensing; pyocyanin
- From: Philippine Journal of Health Research and Development 2023;27(1):45-53
- CountryPhilippines
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVE:Many opportunistic and nosocomial pathogens like Pseudomonas aeruginosa are
very reliant on a bacterium-to-bacterium communication system called quorum sensing (QS). Without the
aforementioned process, gene expressions associated with virulence factors will not be produced. In this study,
the sub-inhibitory concentrations (sub-MICs) of methanolic leaf extract and obtained fractions from Averrhoa
bilimbi (kamias) were screened for ability to inhibit quorum sensing-controlled phenotypes of P. aeruginosa
ATCC 27853.
METHODOLOGY:A. bilimbi crude extract was fractionated through liquid-liquid extraction, producing four (4) fractions: hexane fraction, dichloromethane (DCM) fraction, ethyl acetate (EtOAc) fraction, and water (H2O) fraction. Among the sub-MICs obtained from resazurin-based fluorimetric microtiter assay, only 50 μg/mL was utilized in evaluating the anti-QS properties of crude extract and fr
RESULTS: In the swarming motility assay, hexane fraction (9.39 mm ± 0.67) and DCM fraction (10.82 mm ± 0.95) displayed restriction in the treated P. aeruginosa ATCC 27853 swarms against the control (16.20 mm ± 2.55). In the anti-pyocyanin production assay, hexane fraction exhibited an inhibition of 42.66 % ± 12.94. TLC analysis and phytochemical screening revealed that hexane fraction contains steroids, terpenes, triterpenes, and glycolipids; and DCM fraction contains cardiac glycosides, triterpenoids, terpenes, triterpenes, steroids, alkaloids, and glycolipids.
CONCLUSION:Hexane and DCM fractions obtained from A. bilimbi significantly inhibited swarming of P. aeruginosa ATCC 27853 while none of the extracts were able to significantly inhibit pyocyanin formation of P. aeruginosa ATCC 27853. - Full text:27(1)_5.pdf
