Postmortem Distribution and Postmortem Redistribution of Carbofuran-7-Phenyl Glucuronic Acid in Rabbits.
10.12116/j.issn.1004-5619.2020.301204
- Author:
Yan WEN
1
;
Yu-Zhe HAN
1
;
Duo GONG
1
;
Wen-Kai XIE
1
;
Chen-Xi LÜ
1
;
Yu-Zhen MENG
1
;
Chao ZHANG
1
;
Zhi-Wen WEI
1
;
Ke-Ming YUN
1
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
- Publication Type:Journal Article
- Keywords:
carbofuran-7-phenyl glucuronic acid;
forensic toxicological;
liquid chromatography-tandem mass spectrometry(LC-MS/MS);
postmortem distribution;
postmortem redistribution;
rabbits
- MeSH:
Animals;
Rabbits;
Carbofuran;
Chromatography, Liquid;
Postmortem Changes;
Tandem Mass Spectrometry;
Autopsy
- From:
Journal of Forensic Medicine
2022;38(5):601-605
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To establish a carbofuran intragastric administration death model in rabbits, and to observe the postmortem distribution and postmortem redistribution of carbofuran-7-phenyl glucuronic acid (Glu-7PH) in rabbits.
METHODS:The postmortem distribution: Rabbits were given an administration of 1/2LD50, LD50, 2LD50 carbofuran. Dead rabbits were dissected immediately. Rabbits that had remained alive 2 hours were sacrificed by carbon dioxide (CO2) inhalation and dissected immediately. The myocardium, cardiac blood, liver, spleen, lung, kidney, brain and right hindlimb muscle were collected. The postmortem redistribution: After giving an administration of 4LD50 carbofuran, the myocardium, cardiac blood, liver, spleen, lung, kidney, brain, and right hindlimb muscle were collected at 0, 12, 24, 48, and 72 h postmortem in supine position at 15 ℃ room temperature. The quantity of Glu-7PH was determined by LC-MS/MS.
RESULTS:The postmortem distribution: Among the three dose groups, there were significant differences in the quantities of Glu-7PH in different tissues. The postmortem redistribution: There was no significant difference in the Glu-7PH quantities in cardiac blood, mycardium, spleen, kidney, brain and right hindlimb muscle, but there was a significant difference in the Glu-7PH quantities in the liver and lung.
CONCLUSIONS:The mycardium, cardiac blood, liver, lung, kidney, brain and hindlimb muscle of rabbits can be used as appropriate samples for Glu-7PH detection. However, it should be noted that Glu-7PH was redistributed postmortem in rabbit liver and lung.
