Detection of Synthetic Cannabinoid CUMYL-PEGACLONE in E-Cigarette Oil and Hair.
10.12116/j.issn.1004-5619.2022.320701
- Author:
Tai-Feng JIAO
1
;
Ya-Qing LI
1
;
Gang KANG
1
;
Shen-Shu CHEN
1
;
Liang-Hong CHENG
1
Author Information
1. Guangdong Nantian Institute of Forensic Science, Shenzhen 518003, Guangdong Province, China.
- Publication Type:Journal Article
- Keywords:
CUMYL-PEGACLONE;
forensic toxicology;
gas chromatography-mass spectrometry (GC-MS);
high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS);
synthetic cannabinoid
- MeSH:
Humans;
Illicit Drugs/analysis*;
Tandem Mass Spectrometry;
Electronic Nicotine Delivery Systems;
Cannabinoids;
Hair/chemistry*;
Limit of Detection;
Substance Abuse Detection/methods*
- From:
Journal of Forensic Medicine
2022;38(5):595-600
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To establish a combined high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) method to detect the synthetic cannabinoid CUMYL-PEGACLONE in e-cigarette oil and hair.
METHODS:HPLC-MS/MS and GC-MS were used to establish the detection method of CUMYL-PEGACLONE, and the hair of drug-involved persons and the seized e-cigarette oil were detected.
RESULTS:The main mass spectrometry characteristic ions m/z of CUMYL-PEGACLONE measured by GC-MS were 91, 179, 197, 254 and 372. CUMYL-PEGACLONE had a good linear relationship in the mass concentration range of 2-50 ng/mL, and the linear correlation coefficient (r) was greater than 0.99. The limit of detection (LOD) of CUMYL-PEGACLONE in hair was 0.01 ng/mg, and the limit of quantitation (LOQ) was 0.02 ng/mg. The LOD of CUMYL-PEGACLONE in e-cigarette oil was 1 ng/mg, and the LOQ was 2 ng/mg. The average recoveries of CUMYL-PEGACLONE under the attempt at high, intermediate and low levels in blank human hair and e-cigarette oil matrix were 98.2%-132.4% and 93.5%-110.6%, and the intraday and intraday precision were 1.2%-12.9% and 0.7%-2.9%. CUMYL-PEGACLONE was detected in the hair of 15 drug-involved persons. Except for 1 person who was lower than LOQ, the concentration of CUMYL-PEGACLONE in the hair of other 14 persons was 0.035-0.563 ng/mg. The mass fraction of CUMYL-PEGACLONE in 2 e-cigarette oil were 0.17% and 0.21%, respectively.
CONCLUSIONS:The established HPLC-MS/MS and GC-MS methods are applied to the detection of HPLC-MS/MS in drug-related cases, which provides strong evidence support for the handling authority to quickly investigate these cases, and also provides a reference for the identification of such substances in future.
