Identification of the New Psychoactive Substance Eutylone.
10.12116/j.issn.1004-5619.2021.310503
- Author:
Qian-Ya DENG
1
;
Wen-Juan SUN
1
;
Si-Yang HE
1
;
Kua-Dou WANG
1
;
Yong-Sheng CHEN
1
;
Wei WANG
1
;
Chen LIANG
1
Author Information
1. Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
- Publication Type:Journal Article
- Keywords:
1 H-nuclear magnetic resonance spectroscopy (1 H-NMR);
Eutylone;
Fourier transform infrared spectroscopy (FTIR);
forensic toxicological analysis;
gas chromatography-quadrupole time-of-flight mass spectro-metry (GC-QTOF-MS);
new psychoactive substance;
synthetic cathinones;
ultra-high performance liquid chromatography-linear ion trap quadrupole-orbitrap mass spectrometry (UPLC-LTQ-Orbitrap MS)
- MeSH:
Methanol;
Chromatography, High Pressure Liquid/methods*;
Mass Spectrometry;
Gas Chromatography-Mass Spectrometry/methods*;
Magnetic Resonance Spectroscopy
- From:
Journal of Forensic Medicine
2022;38(4):473-477
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To establish a method to identify unknown sample based on the combined use of Fourier transform infrared spectroscopy (FTIR), gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOF-MS), ultra-high performance liquid chromatography-linear ion trap quadrupole-orbitrap mass spectrometry (UPLC-LTQ-Orbitrap MS) and 1H-nuclear magnetic resonance spectroscopy (1H-NMR) technique.
METHODS:The unknown sample was directly analyzed by FTIR. The unknown sample was dissolved in methanol solution containing internal standard SKF525A and the supernatant was detected by GC-QTOF-MS and UPLC-LTQ-Orbitrap MS. The unknown sample was dissolved in methanol-d4 solution for structural analysis of 1H-NMR.
RESULTS:The characteristic absorption peaks of FTIR spectra obtained from unknown sample were 1 682 (C=O bond), 1 503, 1 488, 1 436, 1 363, 1 256, 1 092, 1 035, 935, 840 and 800 cm-1, the characteristic fragment ions (m/z) of GC-QTOF-MS were 86.096 4 (base peak), 58.065 1, 149.023 5, 121.028 6 and 65.038 6, the accurate mass [M+H]+ detected by UPLC-LTQ-Orbitrap MS was 236.127 7. The sample was identified as synthetic cathinone new psychoactive substance Eutylone by 1H-NMR.
CONCLUSIONS:The method established in this study can be used for structural confirmation of Eutylone.
