Screening Biomarkers of Sudden Coronary Death Based on mRNA Expression Profile of Rat Myocardial Tissues.
10.12116/j.issn.1004-5619.2022.420509
- Author:
Xiang-Jie GUO
1
;
Hao LI
1
;
Ya-Qin BAI
1
;
Peng WU
1
;
Chun-Mei ZHAO
1
;
Yi-Ming DONG
1
;
Nian-Nian CHEN
1
;
Ke-Ming YUN
1
;
Cai-Rong GAO
1
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, Shanxi Province, China.
- Publication Type:Journal Article
- Keywords:
biomarker;
cause of death;
differentially expressed gene;
forensic pathology;
messenger RNA (mRNA);
myocardial ischemia;
rats;
sudden coronary death
- MeSH:
Rats;
Animals;
RNA, Messenger/genetics*;
Gene Regulatory Networks;
Gene Expression Profiling;
Phosphatidylinositol 3-Kinases/genetics*;
Biomarkers
- From:
Journal of Forensic Medicine
2022;38(4):443-451
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To explore the differential expression of messenger RNA (mRNA) in myocardial tissues of rats with sudden coronary death (SCD), and to provide ideas for the forensic identification of SCD.
METHODS:The rat SCD model was established, and the transcriptome sequencing was performed by next-generation sequencing technology. Differentially expressed genes (DEGs) in myocardial tissues of SCD rats were screened by using the R package limma. A protein-protein interaction (PPI) network was constructed by using the STRING database and Cytoscape 3.8.2 on DEG, and hub genes were screened based on cytoHubba plug-in. Finally, the R package clusterProfiler was used to analyze the biological function and signal pathway enrichment of the selected DEG.
RESULTS:A total of 177 DEGs were associated with SCD and were mainly involved in the renin-angiotensin system and PI3K-Akt signaling pathway. The genes including angiotensinogen (AGT), complement component 4a (C4a), Fos proto-oncogene (FOS) and others played key roles in the development of SCD.
CONCLUSIONS:Genes such as AGT, C4a, FOS and other genes are expected to be potential biomarkers for forensic identification of SCD. The study based on mRNA expression profile can provide a reference for forensic identification of SCD.
