Gene Expression Profiles at Different Time Points after Acute Myocardial Infarction in Mice.
10.12116/j.issn.1004-5619.2021.410505
- Author:
Hao LI
1
;
Xiao JIA
2
;
Ya-Qin BAI
1
;
Peng WU
1
;
Hua-Lin GUO
3
;
Ke-Ming YUN
1
;
Cai-Rong GAO
1
;
Xiang-Jie GUO
1
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, Shanxi Province, China.
2. Key Laboratory of Evidence Science, Ministry of Education, China University of Political Science and Law, Beijing 100088, China.
3. Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia, China.
- Publication Type:Journal Article
- Keywords:
acute myocardial infarction;
bioinformatics;
differentially expressed genes;
forensic pathology;
mice;
sequential expression
- MeSH:
Animals;
Computational Biology/methods*;
Gene Expression Profiling/methods*;
Mice;
Mitogen-Activated Protein Kinases/metabolism*;
Myocardial Infarction/metabolism*;
RNA, Messenger;
Ryanodine Receptor Calcium Release Channel/metabolism*;
Transcriptome
- From:
Journal of Forensic Medicine
2022;38(3):343-349
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To explore the mRNA differential expressions and the sequential change pattern in acute myocardial infarction (AMI) mice.
METHODS:The AMI mice relevant dataset GSE4648 was downloaded from Gene Expression Omnibus (GEO). In the dataset, 6 left ventricular myocardial tissue samples were selected at 0.25, 1, 4, 12, 24 and 48 h after operation in AMI group and sham control group, and 6 left ventricular myocardial tissue samples were selected in blank control group, a total of 78 samples were analyzed. Differentially expressed genes (DEGs) were analyzed by R/Bioconductor package limma, functional pathway enrichment analysis was performed by clusterProfiler, protein-protein interaction (PPI) network was constructed by STRING database and Cytoscape software, the key genes were identified by Degree topological algorithm, cluster sequential changes on DEGs were analyzed by Mfuzz.
RESULTS:A total of 1 320 DEGs were associated with the development of AMI. Functional enrichment results included cellular catabolic process, regulation of inflammatory response, development of muscle system and vasculature system, cell adhesion and signaling pathways mainly enriched in mitogen-activated protein kinase (MAPK) signaling pathway. The key genes of AMI included MYL7, TSC22D2, HSPA1A, BTG2, NR4A1, RYR2 were up-regulated or down-regulated at 0.25-48 h after the occurrence of AMI.
CONCLUSIONS:The functional signaling pathway of DEGs and the sequential expression of key genes in AMI may provide a reference for the forensic identification of AMI.
