Establishment of Multiplex Amplification System of STR Loci in Felis Catus and Its Forensic Application.
10.12116/j.issn.1004-5619.2021.511104
- Author:
Shi-Han XI
1
;
Yi-Ling QU
2
;
Ruo-Cheng XIA
2
;
Lei XIONG
2
;
Si-Yu CHAI
2
;
Chun-Lan TONG
1
;
Rui-Yang TAO
2
;
Cheng-Tao LI
1
Author Information
1. Clinical Medical School of Inner Mongolia Minzu University, Tongliao 028000, Inner Mongolia, China.
2. Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
Felis catus;
forensic genetics;
individual identification;
multiplex amplification system;
sex determining region of Y (SRY);
short tandem repeat
- MeSH:
Alleles;
Animals;
Cats/genetics*;
Chromosomes, Human, Y;
DNA Fingerprinting/methods*;
DNA Primers;
Humans;
Microsatellite Repeats/genetics*;
Polymerase Chain Reaction/methods*;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2022;38(2):231-238
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To construct a Felis catus STR loci multiplex amplification system and to evaluate its application value by testing the technical performance.
METHODS:The published Felis catus STR loci data were reviewed and analyzed to select the STR loci and sex identification loci that could be used for Felis catus individual identification and genetic identification. The fluorescent labeling primers were designed to construct the multiplex amplification system. The system was validated for sensitivity, accuracy, balance, stability, species specificity, tissue identity and mixture analysis, and investigated the genetic polymorphisms in 145 unrelated Felis catus samples.
RESULTS:Sixteen Felis catus autosomal STR loci and one sex determining region of Y (SRY) were successfully selected, and constructed a multiplex amplification system containing the above loci. The complete profile of all alleles could still be obtained when the amount of DNA template was as low as 0.25 ng. There was no specific amplification peak in other common animal samples. Population genetic surveys showed that total discrimination power (TDP) of the 16 STR loci was 1-3.57×10-20, the cumulative probability of exclusion (CPE) was 1-6.35×10-5 and the cumulative probability of matching was 3.61×10-20.
CONCLUSIONS:The Felis catus STR multiplex amplification system constructed in this study is highly sensitive, species-specific, and accurate in typing results, which can provide an effective solution for Felis catus species identification, individual identification and kinship identification in the field of forensic science.
