Detection of Exogenous γ-Hydroxybutyric Acid in Rat Blood Exosomes.
10.12116/j.issn.1004-5619.2021.410116
- Author:
Zheng-Xiang GAO
1
;
Qi-Zhi LUO
1
;
Liang ZHANG
2
;
Mao-Qing PEI
2
;
Hui-Jun WANG
1
;
Xia YUE
1
Author Information
1. School of Forensic Medicine, Southern Medical University, Guangzhou 510080, China.
2. Criminal Technology Center, Guangdong Provincial Public Security Department, Guangzhou 510050, China.
- Publication Type:Journal Article
- Keywords:
blood;
exosome;
forensic pathology;
gamma-butyrolactone (GBL);
gamma-hydroxybutyrate (GHB);
rats;
ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS)
- MeSH:
4-Butyrolactone/chemistry*;
Animals;
Chromatography, Liquid;
Exosomes/chemistry*;
Hydroxybutyrates/chemistry*;
Male;
Rats;
Rats, Sprague-Dawley;
Sodium Oxybate/analysis*;
Tandem Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2022;38(2):212-216
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To find a method to distinguish exogenous gamma-hydroxybutyrate (GHB) from endogenous GHB by establishing ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) based on exosome for quantitative detection of GHB in the rat blood.
METHODS:Adult male SD rats were divided into 1 h, 5 h, 10 h administration group and control group. After 1 h, 5 h and 10 h of single precursor of GHB gamma-butyrolactone (GBL) intraperitoneal injection in administration groups, 5 mL blood was collected from the abdominal aorta. Meanwhile, the control group was given a same dose of normal saline, and 5 mL blood was collected at 1 h. Among the 5 mL blood, 0.5 mL was directly detected by HPLC-MS after pretreatment, and exosomes were extracted from the remaining blood by differential centrifugation and detected.
RESULTS:The concentration of GHB in the control group was (87.36±33.48) ng/mL, and the concentration with administration at 1 h, 5 h and 10 h was (110 400.00±1 766.35) ng/mL, (1 479.00±687.01) ng/mL and (133.60±12.17) ng/mL, respectively. The results of exosome detection showed that no peak GHB signal was detected in the control group and the 10 h administration group, and the concentrations of GHB at 1 h and 5 h administration groups were (91.47±33.44) ng/mL and (49.43±7.05) ng/mL, respectively.
CONCLUSIONS:GHB was detected in blood exosome by UPLC-MS, which indicated that exogenous GHB could be detected in plasma exosomes, while endogenous GHB could not be detected, suggesting that this method may be used as a basis to determine whether there is exogenous drug intake.
