Comparison of Application of MD-VF-Auto SEM Method and Plankton Gene Multiplex PCR System in the Diagnosis of Drowning.
10.12116/j.issn.1004-5619.2021.410605
- Author:
Jian-Miao ZHANG
1
;
Tian-Chun LIN
1
;
Zhen-Yu LIAO
1
;
Yu-Kun DU
2
;
Zhong-Hao YU
2
;
Jing-Jian LIU
3
;
Sai-Qun WU
4
;
Xiao-Dong KANG
5
;
Qu-Yi XU
5
;
He SHI
5
;
Jian ZHAO
5
;
Chao LIU
5
;
Dang-En GU
1
Author Information
1. Huadu District Branch of Guangzhou Public Security Bureau, Guangzhou 510800, China.
2. School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
3. College of Forensic Medicine, Kunming Medical University, Kunming 650500, China.
4. Guangdong Provincial Public Security Department, Guangzhou 510030, China.
5. Guangzhou Forensic Science Institute & Key Laboratory of Forensic Pathology, Ministry of Public Security, Guangzhou 510442, China.
- Publication Type:Journal Article
- Keywords:
diatom test;
drowning;
forensic pathology;
microwave digestion-vacuum filtration-automated scanning electron microscopy method;
multiplex PCR system;
plankton
- MeSH:
Diatoms/genetics*;
Drowning/diagnosis*;
Humans;
Liver;
Lung;
Microscopy, Electron, Scanning;
Multiplex Polymerase Chain Reaction;
Plankton/genetics*
- From:
Journal of Forensic Medicine
2022;38(1):114-118
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To compare the application effect of microwave digestion - vacuum filtration - automated scanning electron microscopy (MD-VF-Auto SEM) method and plankton gene multiplex PCR system in the diagnosis of drowning.
METHODS:Lung, liver and kidney tissue of 10 non-drowning cases and 50 drowning cases were prepared for further MD-VF-Auto SEM method analysis and plankton gene multiplex PCR system analysis. The positive detection rate of the two methods in each tissue was calculated.
RESULTS:The positive rate of the MD-VF-Auto SEM method detecting diatoms in drowning cases was 100%, and few diatoms were detected in the liver and kidney tissues of 6 non-drowning cases. By using the plankton gene multiplex PCR system, the diatom positive rate of drowning cases was 84%, and all the non-drowning cases were negative. There were significant differences in the positive rate of the liver, kidney tissues between MD-VF-Auto SEM method and plankton gene multiplex PCR system (P<0.05), as well as the total positive rate of cases. However, no significant differences were found in the positive rates of lung tissues (P>0.05).
CONCLUSIONS:MD-VF-Auto SEM method is more sensitive than plankton gene multiplex PCR system in diatom test. But the plankton gene multiplex PCR system can also detect plankton other than diatoms. Combination of the two methods can provide a more reliable basis for the diagnosis of drowning.
