Effects of Digestive Temperature and Time on Diatom Test.
10.12116/j.issn.1004-5619.2021.410611
- Author:
Jing-Jian LIU
1
;
Yu-Kun DU
2
;
Jian ZHAO
3
;
Xiao-Dong KANG
3
;
Zhong-Hao YU
2
;
Dong-Yun ZHENG
3
;
He SHI
3
;
Qu-Yi XU
3
;
Li-Fang CHEN
1
;
Chao LIU
3
Author Information
1. College of Forensic Medicine, Kunming Medical University, Kunming 650500, China.
2. School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
3. Guangzhou Forensic Science Institute & Key Laboratory of Forensic Pathology, Ministry of Public Security, Guangzhou 510442, China.
- Publication Type:Journal Article
- Keywords:
diatom test;
digestive temperature;
digestive time;
forensic pathology
- MeSH:
Diatoms;
Drowning;
Forensic Pathology/methods*;
Temperature;
Water
- From:
Journal of Forensic Medicine
2022;38(1):77-81
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To study the effects of temperature and time for diatoms digestion and find out suitable digestive temperature and time.
METHODS:Eighty pieces of liver tissues were collected, each piece of tissue was 2 g, and 2 mL Pearl River water was added to each piece of tissue. The digestion temperature was set at 100 ℃, 120 ℃, 140 ℃, 160 ℃, 180 ℃ and the digestion time was set at 40, 50, 60, 70, 80 min. The liver tissue and water mixture were divided into 8 portions in each group. All the samples were tested by microwave digestive - vacuum filtration - automated scanning electron microscopy method. The quantity of diatom recovered and the quality of residue on the membrane were recorded.
RESULTS:When the digestion time was set to 60 min, there were statistically significant differences in the number of diatoms recovered at different temperatures (P<0.05). The maximum number of diatoms recovered was (28 797.50±6 009.67) at 140 ℃, and the minimum residue was (0.60±0.28) mg at 180 ℃. When the digestion temperature was set at 140 ℃, there were statistically significant differences in the number of diatoms recovered at different digestion times (P<0.05). The number of diatoms recovered was the highest at 40 min, it was up to (20 650.88±1 950.29), and the residue quality of each group had no statistical significance among different digestion time groups(P>0.05).
CONCLUSIONS:The effect of diatom digestion is related to temperature and time. When the digestion temperature was 140 ℃ and the digestion time was 40, 50 and 60 min, it is favorable for diatom test.
