Forensic Application of ForenSeqTM DNA Signature Prep Kit in Zhengjiang She Ethnic Group.
10.12116/j.issn.1004-5619.2021.510404
- Author:
Yi-Ling QU
1
;
Yuan LIN
2
;
Zi-Hao YANG
2
;
Rui-Yang TAO
2
;
Ruo-Cheng XIA
2
;
Zheng-Jun CAO
3
;
Rui-Xiang GAO
3
;
Huan YU
1
;
Zi-Wei WANG
1
;
Qi YANG
1
;
Xiao-Chun ZHANG
1
;
Su-Hua ZHANG
2
;
Cheng-Tao LI
1
Author Information
1. School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, China.
2. Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
3. Criminal Police Detachment of Yancheng Public Security Bureau, Yancheng 224000, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
ForenSeq TM DNA Signature Prep kit;
She ethnic group;
Zhejiang;
forensic genetics;
massively parallel sequencing;
polymorphism, genetic;
short tandem repeat (STR)
- MeSH:
DNA;
DNA Fingerprinting/methods*;
Ethnicity/genetics*;
Gene Frequency;
High-Throughput Nucleotide Sequencing/methods*;
Humans;
Male;
Microsatellite Repeats;
Polymorphism, Single Nucleotide;
Sequence Analysis, DNA/methods*
- From:
Journal of Forensic Medicine
2021;37(6):817-824
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To evaluate the ability of the ForenSeqTM DNA Signature Prep kit (ForenSeq kit) in analyzing the sequence information of STRs in Zhejiang She ethnic group and its forensic application efficacy.
METHODS:A total of 50 Zhejiang She ethnic group samples were sequenced with the ForenSeq kit on the MiSeq FGx platform. The data was analyzed using ForenSeqTM universal analysis software to obtain the motif structure and flank regions of the 58 STRs, then compared with PCR-CE typing results to test the consistency. At last, the allele frequency and population genetic parameters were calculated.
RESULTS:A total of 448 sequence polymorphic alleles were detected in 50 samples of Zhejiang She ethnic group. Compared with fragment length polymorphism detected by PCR-CE, 82 alleles were increased by MPS detection based on ForenSeq kit, and 7 SNPs variation were detected in the flanking regions of 6 loci. The 22 male individuals were genotyped, and total 19 haplotypes were detected in 24 Y chromosome STRs of these 22 males. The cumulative discrimination power of the 27 autosomal STRs was 1-8.87×10-30, the cumulative probability of exclusion of duo-testing was 0.999 999 962 640 657, the cumulative probability of exclusion of trios-testing was 0.999 999 999 999 633.
CONCLUSIONS:Based on MPS typing technology, using the ForenSeq kit greatly improves the detection efficiency. In addition, the 58 STRs have good genetic polymorphisms in Zhejiang She ethnic group, which are suitable for individual identification and paternity identification in forensic application.
