Development and Forensic Application of EX20+30Y Kit.
10.12116/j.issn.1004-5619.2020.500509
- Author:
Xu-Ming ZHOU
1
;
Deng-Xing GAO
2
;
Fa-Yuan LI
3
Author Information
1. Expert Evidence Center of Shangrao Public Security, Shangrao 334000, Jiangxi Province, China.
2. Expert Evidence Center of Xinyu Public Security, Xinyu 338000, Jiangxi Province, China.
3. AGCU ScienTech Incorporation, Wuxi 214174, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
Y chromosome;
autosome;
forensic genetics;
kit;
short tandem repeat
- MeSH:
Chromosomes, Human, Y/genetics*;
DNA Fingerprinting/methods*;
Forensic Medicine/methods*;
Humans;
Microsatellite Repeats;
Multiplex Polymerase Chain Reaction;
Species Specificity
- From:
Journal of Forensic Medicine
2021;37(5):639-645
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To develop a multiplex PCR amplification system (EX20+30Y for short) of 19 autosomes, 30 Y-STR loci plus the gender indicator, and evaluate its forensic application value.
METHODS:With the six-color fluorescence labeling technology, a multiplex amplification system of 19 autosomal STR loci and 30 Y-STR loci plus the gender indicator was constructed. Blood samples from 210 unrelated individuals, 69 daily case samples and standard samples 9948 and 9947A were collected for loci detection and analysis. The EX20+30Y multiplex amplification system was evaluated by its sensitivity, mixed sample detection ability, species specificity, balance, direct amplification ability, sample applicability and anti-inhibition ability.
RESULTS:Multiplex amplification of blood samples from 210 unrelated individuals by the detection system obtained accurate genotyping results. The detection sensitivity of standard samples was 0.125 ng and the species specificity was high. The 69 samples from daily cases were genotyped correctly. Moreover, standard sample 9948 could be accurately genotyped even if the samples contained a certain concentration of inhibitors.
CONCLUSIONS:The multiplex amplification system established in this study can conduct combined examination of 19 autosomes, 30 Y-STR loci plus the gender indicator with accurate genotyping and high sensitivity. It has a good forensic application prospect.
