Bacterial Succession on Rat Carcasses and Applications for PMI Estimation.
- Author:
Lin ZHANG
;
Juan-juan GUO
;
TELET-SIYIT
;
Yu-long PENG
;
Dan XIE
;
Ya-dong GUO
;
Jie YAN
;
Lagabaiyila ZHA
;
Ji-feng CAI
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Autopsy;
Cadaver;
Death;
Forensic Medicine/methods*;
Neisseria lactamica;
Postmortem Changes;
Rats;
Rats, Sprague-Dawley;
Staphylococcus aureus;
Time Factors
- From:
Journal of Forensic Medicine
2016;32(1):1-6
- CountryChina
- Language:English
-
Abstract:
UNLABELLED:Abstract:
OBJECTIVE:To investigate the bacterial succession on rat carcasses and to evaluate the use of bacterial succession for postmortem interval (PMI) estimation.
METHODS:Adult female SD rat remains were placed in carton boxes. The bacterial colonization of circumocular skin, mouth and vagina was collected to be identified using culture-dependent biochemical methods. The changes in community composition were regularly documented.
RESULTS:The bacterial succession in three habitats showed that Staphylococcus and Neisseria were predominated in early PMI, especially Staphylococcus aureus and Neisseria lactamica in 6 hours after death. Lactobacillus casei developed on the 3-4 days regularly, and kept stable at a certain level in late PMI.
CONCLUSION:The involvement of normal and putrefactive bacteria in three body habitats of rat remains can be used for PMI estimation.
