Proteomic Analysis of Rat Brain Stem with DAI by MALDI-TOF-MS.
- Author:
Guan-heng REN
;
Ning-guo LIU
;
Yi-jiu CHEN
;
Yan SHI
;
Dong-hua ZOU
;
Ping HUANG
;
Zheng-dong LI
;
Ho Yu SHA
;
Kai-fei DENG
- Publication Type:Journal Article
- MeSH:
Animals;
Biomarkers;
Brain Stem/metabolism*;
Diffuse Axonal Injury/diagnosis*;
Down-Regulation;
Male;
Peptides/blood*;
Proteomics;
Rats;
Rats, Sprague-Dawley;
Sensitivity and Specificity;
Software;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*;
Up-Regulation
- From:
Journal of Forensic Medicine
2016;32(1):13-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a diagnostic model for diffuse axonal injury (DAI) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). To screen the proteins or peptides associated with DAI for providing the biomarkers with theoretic foundation.
METHODS:Fifteen male Sprague-Dawley rats were randomly divided into DAI group (n = 10) and control group (n = 5). The protein or peptide expression profiles of rat brain stem were detected by MALDI-TOF-MS. ClinProTools 2.2 software was used to find specific peaks, and a diagnostic model was established by the genetic algorithm.
RESULTS:There were significant differences in 61 peaks of DAI group (P < 0.05), 9 peaks were down-regulated and 52 up-regulated. The diagnostic model was established based on 5 different peaks. The specificity and sensitivity of cross validation was 96.14% and 95.98%; while the specificity and sensitivity of blind validation showed was 73.33% and 70.00%, respectively.
CONCLUSION:A specific and sensitive diagnostic model of DAI can be established by MALDI-TOF-MS to provide a potential value for determining DAI in forensic practice.
