Effects of PI3K/Akt Pathway in Wound Healing Process of Mice Skin.
- Author:
Yan-ling GAO
;
Chang-sheng LIU
;
Rui ZHAO
;
Lin-lin WANG
;
Shan-shan LI
;
Min LIU
;
Miao ZHANG
;
Shu-kun JIANG
;
Zhi-ling TIAN
;
Meng WANG
;
Da-wei GUAN
- Publication Type:Journal Article
- MeSH:
Animals;
Blotting, Western;
Class I Phosphatidylinositol 3-Kinases;
Fibroblasts/metabolism*;
Mice;
Phosphatidylinositol 3-Kinase;
Phosphatidylinositol 3-Kinases/metabolism*;
Phosphorylation;
Proto-Oncogene Proteins c-akt/metabolism*;
Real-Time Polymerase Chain Reaction;
Signal Transduction;
Skin/injuries*;
Wound Healing
- From:
Journal of Forensic Medicine
2016;32(1):7-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expressions and time-dependent changes of phosphatidylinositol-3-kinase (PI3K), phospho-PI3K (p-PI3K), protein kinase B (PKB/Akt) and phospho-Akt (p-Akt) during wound healing process of mice skin.
METHODS:The changes of PI3K, p-PI3K, Akt and p-Akt expression in skin wound were detected by immunohistochemistry, Western blotting and real-time PCR.
RESULTS:Immunohistochemistry showed the expression of PI3K and p-Akt were observed in mononuclear and fibroblast after skin wound, and reached peak in reconstruction. The positive bands of PI3K, p-PI3K, Akt and p-Akt were observed in all time points of the wound healing process by Western blotting. The expression peak of p-PI3K and p-Akt showed in inflammation and proliferation; the expression peak of PI3K and Akt in reconstruction. Real-time PCR showed the expression peak of PI3K mRNA in inflammation and reconstruction and the peak of Akt mRNA in reconstruction.
CONCLUSION:During the wound healing process, the expressions of PI3K, Akt, p-PI3K and p-Akt show different changes with significant correlation to wound time. The expression of PI3K/Akt may be a valuable marker for wound time estimation.
