Establishing a 29 Y-STR Loci Multiplex PCR System.
- Author:
Xin-jie WANG
;
Li-jing LUO
;
Lei HUANG
;
Xin XU
- Publication Type:Journal Article
- MeSH:
Asian People/genetics*;
China;
Chromosomes, Human, Y;
DNA/isolation & purification*;
Ethnicity/genetics*;
Forensic Genetics/methods*;
Forensic Sciences;
Genetics, Population/methods*;
Haplotypes;
Humans;
Male;
Microsatellite Repeats;
Multiplex Polymerase Chain Reaction/methods*;
Polymorphism, Genetic;
Reproducibility of Results
- From:
Journal of Forensic Medicine
2015;31(6):456-461
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a 29 Y-STR loci multiplex PCR system for investigating the genetic polymorphisms and to assess its application value in forensic science.
METHODS:A multiplex PCR system was established using a five color fluorescence labeling 29 Y-STR loci (DYS456, DYS389 I , DYS437, DYS447, DYS389 11, DYS438, DYS522, DYS460, DYS458, DYS622, DYS390, DYS392, DYS448, DYS449, DYS391, Y-GA TA-H4, DYS388, DYS19, DYS385a/b, DYS527a/b, DYS393, DYS459a/b, DYS635, DYS439, DYS570 and DYS627) for multiple amplification and capillary electrophoresis. And its applicability was validated with genetic polymorphism data of 29 Y-STR of unrelated 2,000 male samples in Shandong Han population.
RESULTS:A total of 1,981 different haplotypes of 2,000 individuals showed genotype diver- sity between 0.370 0 and 0.965 4. The system provided stable and accurate typing with high sensitivity of 0.05 ng. It satisfied the needs of variety of routine biological samples.
CONCLUSION:The 29 Y-STR loci multiplex PCR system could be applied for actual cases and establishment of Y-STR database. In addition, it has great significance in forensic science practices and related research.
