DNA Extraction of Cast-off Cells of Fingerprints from 502 Glue Fumigated Contact Samples.
- Author:
Xian-wen WANG
;
Xue-feng LENG
;
Shou-yu WANG
- Publication Type:Journal Article
- MeSH:
Adhesives;
DNA/isolation & purification*;
DNA Fingerprinting/methods*;
Forensic Medicine;
Fumigation/methods*;
Humans
- From:
Journal of Forensic Medicine
2015;31(6):454-461
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method of fingerprint position, sample transfer and fingerprint DNA extraction in contact samples.
METHODS:Sixty-six cases were visualized by 502 glue fingerprint fumigation. Two methods, ordinary wipe and acetone wipe, were used to transfer cast-off cells of fingerprints from testing samples, respectively. DNA was extracted and purified by ultramicro magnetic bead kit. The data was resolved on genetic analysis after amplification.
RESULTS:In 33 samples, 30 samples got better STR analysis by acetone wipe method. The peak range was 1,000-4,000 RFU and peak shapes were equable. It was hard to get ideal STR typing by ordinary wipe method.
CONCLUSION:The samples are visualized by 502 glue fingerprint fumigation and the case-off cells are transferred by acetone wipe method. The method shows better STR analysis result, which might be a better method for forensic science practice.
