Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci.
- Author:
Yan DONG
;
Shuang-shuang LIN
;
Yu CAO
;
Wei-wei WU
;
Shu-qin HUANG
;
Wei-guo ZHENG
;
Fa-yuan LI
;
Bin-wen GE
;
Yu-lin GUO
;
Huai-gu ZHOU
- Publication Type:Journal Article
- MeSH:
Amelogenin;
Chromosomes, Human, Y/genetics*;
DNA Primers;
Databases, Nucleic Acid;
Forensic Genetics/methods*;
Genotype;
Genotyping Techniques/instrumentation*;
Humans;
Indicators and Reagents;
Microsatellite Repeats;
Multiplex Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2015;31(5):373-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.
METHODS:Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.
RESULTS:A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin.
CONCLUSION:The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.
