Forensic Validation of the Goldeneye™ DNA ID 25A Kit.
- Author:
Yao-dong SUN
;
Li-ping CAO
- Publication Type:Journal Article
- MeSH:
Asian People/genetics*;
China;
Chromosomes, Human, Y;
DNA/genetics*;
DNA Fingerprinting/standards*;
Databases, Nucleic Acid;
Female;
Forensic Genetics/methods*;
Genotype;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Reproducibility of Results;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2015;31(4):284-286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To test and estimate the forensic application of Goldeneye™ DNA ID 25A Kit.
METHODS:The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework.
RESULTS:The peak height balance of the different loci was ≥ 42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA.
CONCLUSION:Goldeneye™ DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.
