Fourier Transform Infrared Microspectroscopy of Rat Kidney with Regard to Fa- tal Hyperthermia.
- Author:
Zhi-jun WANG
;
Shan-shan SHEN
;
Kai-fei DENG
;
Zhi-qiang QIN
;
Ping HUANG
;
Zhen-yuan WANG
- Publication Type:Journal Article
- MeSH:
Animals;
Fever/mortality*;
Fourier Analysis;
Kidney/metabolism*;
Microspectrophotometry;
Rats;
Spectroscopy, Fourier Transform Infrared/methods*
- From:
Journal of Forensic Medicine
2015;31(4):257-261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the chemical groups changing in rat kidney with regard to fatal hyperthermia by Fourier transform infrared microspectroscopy (FTIR-MSP) and to provide a new method to diagnose fatal hyperthermia.
METHODS:Rats were sacrificed by hyperthermia, brainstem injury, massive hemorrhage and asphyxiation and divided into groups. The renal samples were dissected immediately after death. The data of infrared spectroscopy in glomerulus were measured by FTIR-MSP.
RESULTS:The absorbances of 3290, 3070, 2850, 1540 and 1396 cm(-1) significantly increased (P < 0.05), and the ratios of Al650/A3290 and A1650/A1540 significantly decreased (P < 0.05) in group of hyperthermia.
CONCLUSION:FTIR-MSP can analyze the changes of chemical groups of kidney as an auxiliary diagnosis for discriminating hyperthermia with other causes of death.
