Expression of Zonula Occludens-1 in Cerebral Cortex Following Traumatic Brain Injury.
- Author:
Tao WANG
;
Ying MENG
;
Dong-hua ZOU
;
Zheng-dong LI
;
Yi-jiu CHEN
;
Lu-yang TAO
- Publication Type:Journal Article
- MeSH:
Animals;
Blood-Brain Barrier;
Blotting, Western;
Brain Injuries/physiopathology*;
Cerebral Cortex/metabolism*;
Immunohistochemistry;
Mice;
Permeability;
Tight Junctions/metabolism*;
Zonula Occludens-1 Protein/metabolism*
- From:
Journal of Forensic Medicine
2015;31(2):85-92
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the time-course expression of zonula occludens-1 (ZO-1) in cerebral cortex after traumatic brain injury (TBI).
METHODS:The TBI model of mouse was established. The mice were divided in 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, 7 d after TBI, sham and control groups. The permeability of the blood brain barrier was evaluated by measuring the extravasation of Evans blue (EB) dye. The expression of ZO-1 in cerebral cortex in the injured area was detected by Western blotting and immunohistochemistry.
RESULTS:The extravasation of EB dye of injured cortex gradually increased from 1 h, peaked at 1-3 d and approximately decreased to normal at 7 d after TBI. Western blotting revealed that the expression of ZO-1 gradually decreased after 1 h, was at the lowest at 1-3 d, and then significantly increased after 7 d but was still lower than that of normal and sham groups. The result of immunohistochemistry showed that ZO-1 had strong expression in vessel of normal cortex, gradually decreased after TBI, and almost disappeared at 3 d after TBI and gradually recovered to normal level later.
CONCLUSION:The expression of ZO-1 in the injured cortex after TBI initially decreases and then increases. The negative correlation between ZO-1 expression and EB extravasation after TBI could be used as a new indicator for wound age estimation.
