Simultaneous screening for 45 poisonous alkaloids in blood by LC-MS/MS.
- Author:
Jin-Xiao ZHAI
;
Min SHEN
;
Wei LIU
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alkaloids/chemistry*;
Chromatography, Liquid/methods*;
Forensic Medicine;
Humans;
Liquid-Liquid Extraction;
Mass Spectrometry;
Sensitivity and Specificity;
Tandem Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2015;31(1):28-33
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening method for 45 poisonous alkaloids in blood.
METHODS:Identification was based on the compound's retention time and two precursor-to-production transitions. The method involved a liquid-liquid extraction (LLE) followed by LC-MS/MS with multiple-reaction monitoring (MRM). When 1 mL of blood was extracted with diethyl ether at pH = 9.2 with SKF525A as the internal standard, the target compounds were analyzed with LC-MS/MS in the positive ionization mode.
RESULTS:The target alkaloids had good linearity (r>0.995 1), both the intra-day precision and inter-day precision being less than 14.77%. The limits of detection ranged from 0.05 to 25 ng/mL in blood.
CONCLUSION:The method is selective and sensitive in detecting poisonous alkaloids with a total running time of 12 minutes; therefore it was successfully applied to some actual cases of suspected alkaloids poisoning.
