Analysis of pesticides in blood specimen by GC/MS with accelerated solvent extraction.
- Author:
Wen-hai LI
;
Da-wei LIN
;
Hong-lei SUN
;
Maimaitituxun MUTAILIFU
;
Lei WANG
- Publication Type:Journal Article
- MeSH:
Chromatography, Liquid;
Gas Chromatography-Mass Spectrometry;
Pesticides/blood*;
Solvents;
Temperature;
Time Factors
- From:
Journal of Forensic Medicine
2014;30(6):463-465
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop the accelerated solvent extraction (ASE) for determining pesticides present in blood samples.
METHODS:Pesticides were extracted by ASE with optimized parameters to study recovery rate affected by extraction temperature, time and agent. GC/MS was used to perform quantitative analysis.
RESULTS:The recovery rates of eight pesticides were 70.6%-92.4%. The coefficient of variation was less than 5.0%. A good linear relationship was obtained at the concentration range of 0.5-5.0 microg/mL.
CONCLUSION:The method was fast and simple with high recovery rate and good repeatability. It can be applied to analyze pesticides present in the blood specimen.
