Effects of additives in blood collection tubes on testing the alcohol concentration in blood samples.
- Author:
Dong-xian LIU
;
Jiang-nan HE
- Publication Type:Journal Article
- MeSH:
Anticoagulants;
Blood Specimen Collection/methods*;
Citrates;
Ethanol/blood*;
Humans;
Sodium Citrate
- From:
Journal of Forensic Medicine
2014;30(6):452-455
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To discuss blood collection tubes with different additives and their effects on the testing results of alcohol concentration in blood samples.
METHODS:Blood samples from 10 volunteers were collected 2 hours after drinking with seven different types of disposable vacuum blood collection tubes, including ordinary tube without anticoagulant, coagulant tube, separating gel-coagulant tube, sodium citrate (1:4) tube, sodium citrate (1:9) tube, sodium citrate (9:1) tube and EDTA-K2 tube. The alcohol concentrations in these blood samples were analyzed by headspace gas chromatography.
RESULTS:The concentration testing results of the same blood samples in different types of tubes were different from one to another. The sequence was as follows: separating gel-coagulant tube > coagulant tube > ordi- nary tube without anticoagulant > EDTA-K2 tube> sodium citrate (1:9) tube> sodium citrate (1:4) tube, whereas the results of the same blood sample in sodium citrate (1:9) tube and sodium citrate (9:1) tube showed no obvious difference.
CONCLUSION:It is better to collect a suspicious drunk driver's blood sam- ple using a disposable vacuum blood collection tube, with the EDTA-K2 tube being preferred.
