Allele-specific PCR and its application in forensic science.
- Author:
Yan-chai NIE
;
Bin WANG
;
Zi-qin ZHAO
;
Huai-gu ZHOU
- Publication Type:Journal Article
- MeSH:
Alleles;
DNA Primers;
Forensic Sciences;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Polymorphism, Single Nucleotide
- From:
Journal of Forensic Medicine
2014;30(4):282-287
- CountryChina
- Language:Chinese
-
Abstract:
Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular diagnosis, and forensic biological evidence. The article systematically reviews the principle, the detection methods, improvement of AS-PCR, and its research updates in the fields of autosome, Y chromosome and mitochondrial SNP, as well as its application in forensic science.
