Cathepsin L expression in plasma after acute myocardial ischemia and ischemia-reperfusion in rats.
- Author:
Geng-qian ZHANG
;
Zheng LIANG
;
Peng YAN
;
Xiao-jia ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Biomarkers/blood*;
Cathepsin L/analysis*;
Isoflurane;
Myocardial Infarction/metabolism*;
Myocardial Ischemia;
Myocardial Reperfusion Injury/metabolism*;
Myocardium;
Rats
- From:
Journal of Forensic Medicine
2014;30(4):253-256
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To test cathepsin L as a biomarker of myocardial ischemia by examination of cathepsin L expression in plasma after myocardial ischemia and ischemia-reperfusion in rats.
METHODS:The rat models were established and divided in acute myocardial ischemia model (myocardial ischemia 30 min, 1 h, 2 h groups), ischemia-reperfusion model (ischemia-reperfusion group), and isoflurane-pretreated ischemia-reperfusion model (isoflurane-pretreated group), respectively. Normal control group and sham-operated group were established as contrast. The contents of cathepsin L in plasma were examined by ELISA and myocardial infarction areas were measured after TTC staining.
RESULTS:No statistical significant changes were found among the experimental groups compared with the normal control group and sham-operated group (P>0.05). The cathepsin L from the ischemia-reperfusion group increased to 2.37 times compared with the normal control group (P<0.05). The cathepsin L and myocardium infarction size of isoflurane-pretreated group decreased compared with the ischemia-reperfusion group (P<0.05).
CONCLUSION:The cathepsin L in plasma is not a promising biomarker of acute myocardial ischemia. Isoflurane preconditioning can reduce the cathepsin L in plasma caused by ischemia-reperfusion injury.
