Nitric oxide mediated TNF-α, IL-1β gene expression in liver induced by crush injury of rat's soft tissues.
- Author:
Guo-kai DONG
;
Xiao-tong ZHANG
;
Li-qin MA
;
Na LI
;
Chun-ling MA
;
Bin CONG
;
Zhen-yong GU
- Publication Type:Journal Article
- MeSH:
Animals;
Gene Expression;
Interleukin-1beta/metabolism*;
Liver;
Nitric Oxide/physiology*;
RNA, Messenger;
Rats;
Tumor Necrosis Factor-alpha/metabolism*;
Wounds and Injuries
- From:
Journal of Forensic Medicine
2014;30(4):250-256
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of nitric oxide (NO) on the gene expression of hepatic TNF-α and IL-1β by crush injury of rat's soft tissues.
METHODS:Rats were randomly divided into sham group, crush group, crush+aminoguanidine (AG) group, and crush+L-arginine (L-Arg) group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1β were detected with RT-PCR.
RESULTS:Obvious increase in TNF-α and IL-1β mRNA expression was detected in the crush group compared with the sham group (P<0.05). After pretreated L-Arg, expressions of TNF-α and IL-1β mRNA were markedly increased (P<0.05). After pretreated AG, those indices obviously decreased (P<0.05). Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group (P<0.05). Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively.
CONCLUSION:Endogenous NO mediated TNF-α, IL-1β mRNA up expression in liver induced by increased production of NO after crush injury of rat's soft tissues.
