Consistency study of PowerPlex 21 kit and Goldeneye 20A kit and forensic application.
- Author:
He REN
;
Ying LIU
;
Qing-Xia ZHANG
;
Zhang-Ping JIAO
- Publication Type:Validation Study
- MeSH:
DNA/analysis*;
DNA Fingerprinting;
DNA Primers;
Genotype;
Heterozygote;
Humans;
Microsatellite Repeats;
Paternity;
Polymerase Chain Reaction;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2014;30(3):191-193
- CountryChina
- Language:Chinese
-
Abstract:
UNLABELLED:To ensure the consistency of genotype results for PowerPlex 21 kit and Goldeneye 20A kit.
METHODS:The STR loci were amplified in DNA samples from 205 unrelated individuals in Beijing Han population. And consistency of 19 overlap STR loci typing were observed. The genetic polymorphism of D1S1656 locus was obtained.
RESULTS:All 19 overlap loci typing showed consistent. The proportion of peak height of heterozygous loci in two kits showed no statistical difference (P > 0.05). The observed heterozygosis of D1S1656 was 0.878. The discrimination power was 0.949. The excluding probability of paternity of triplet was 0.751. The excluding probability of paternity of diploid was 0.506. The polymorphism information content was 0.810.
CONCLUSION:PowerPlex 21 kit and Goldeneye 20A kit present a good consistency. The primer design is reasonable. The polymorphism of D1S1656 is good. The two kits can be used for human genetic analysis, paternity test, and individual identification in forensic practice.
