DNA quantification of blood samples pre-treated with pyramidon.
- Author:
Chuan-Hong ZHU
;
Dao-Li ZHENG
;
Rao-Zhi NI
;
Hai-Sheng WANG
;
Ping NING
;
Hui FANG
;
Yan LIU
- Publication Type:Journal Article
- MeSH:
Aminopyrine/pharmacology*;
Blood Stains;
DNA/isolation & purification*;
DNA Fingerprinting;
Forensic Medicine;
Humans;
Polymerase Chain Reaction;
Reproducibility of Results;
Specimen Handling
- From:
Journal of Forensic Medicine
2014;30(3):188-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study DNA quantification and STR typing of samples pre-treated with pyramidon.
METHODS:The blood samples of ten unrelated individuals were anticoagulated in EDTA. The blood stains were made on the filter paper. The experimental groups were divided into six groups in accordance with the storage time, 30 min, 1 h, 3 h, 6 h, 12 h and 24h after pre-treated with pyramidon. DNA was extracted by three methods: magnetic bead-based extraction, QIAcube DNA purification method and Chelex-100 method. The quantification of DNA was made by fluorescent quantitative PCR. STR typing was detected by PCR-STR fluorescent technology.
RESULTS:In the same DNA extraction method, the sample DNA decreased gradually with times after pre-treatment with pyramidon. In the same storage time, the DNA quantification in different extraction methods had significant differences. Sixteen loci DNA typing were detected in 90.56% of samples.
CONCLUSION:Pyramidon pre-treatment could cause DNA degradation, but effective STR typing can be achieved within 24 h. The magnetic bead-based extraction is the best method for STR profiling and DNA extraction.
