Expression of CaMK II delta in cerebral cortex following traumatic brain injury.
- Author:
Hong PAN
;
Jing-Jing ZHANG
;
Dong-Dong XU
;
Zhen-Yong GU
;
Lu-Yang TAO
;
Ming-Yang ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Blotting, Western;
Brain Injuries/metabolism*;
Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism*;
Cerebral Cortex/metabolism*;
Forensic Medicine;
Immunohistochemistry;
Rats;
Time Factors
- From:
Journal of Forensic Medicine
2014;30(3):169-177
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the time-course expression of calcium-calmodulin dependent protein kinase II delta (CaMK II delta) in cerebral cortex after traumatic brain injury (TBI).
METHODS:The TBI rat model was established. The expression of CaMK II delta in cerebral cortex around injured area was tested by Western blotting and immunohistochemical staining.
RESULTS:Western blotting revealed expression of CaMK II delta in normal rat brain cortex. It gradually increased after TBI, peaked after 3 days, and then returned to normal level. The result of immunohistochemical staining was consistent with that of Western blotting.
CONCLUSION:The expression of CaMK II delta around injured area after TBI increased initially and then decreased. It could be used as a new indicator for wound age determination following TBI.
