Correlation between percentages of PMN, MNC, FBC and wound age after skeletal muscle injury in rats.
- Author:
Tian-Shui YU
;
Da-Wei GUAN
;
Rui ZHAO
;
Hai-Dong ZHANG
;
Ru-Feng BAI
- Publication Type:Journal Article
- MeSH:
Animals;
Fibroblasts;
Muscle, Skeletal/injuries*;
Neutrophils;
Rats;
Time Factors
- From:
Journal of Forensic Medicine
2014;30(3):166-168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the percentages of polymorphonuclear leukocytes (PMN), mononuclear cells (MNC) and fibroblastic cells (FBC) in different post-traumatic intervals after skeletal muscle mechanical injury in rats.
METHODS:The rat model of skeletal muscle mechanical injury was established. The rats were divided into injured groups (6 h, 12 h, 1 d, 3 d, 7 d, 10 d and 14 d after injury) and control group. The percentages of PMN, MNC and FBC in different post-traumatic intervals after skeletal muscle mechanical injury were assessed with HE staining and image analysis.
RESULTS:At post-injury 6-12h, the percentages of PMN and MNC infiltration appeared in injured sites and that of PMN reached peak. At 1 d, the percentage of MNC infiltration appeared and reached peak, while that of PMN decreased. At 3-7 d, the percentage of FBC gradually increased, while that of PMN and MNC decreased. At 10-14d, the percentage of FBC reached peak.
CONCLUSION:The percentages of PMN, MNC and FBC in injured zones showed time-dependent changes, which might be used as reference index for determination of age of skeletal muscle injury.
