Development of an 18 X-InDel multiplex PCR system.
- Author:
Kuan SUN
;
Shu-Min ZHAO
;
Su-Hua ZHANG
;
Cheng-Tao LI
- Publication Type:Journal Article
- MeSH:
Amelogenin/genetics*;
Asian People;
DNA Fingerprinting;
DNA Primers;
Ethnicity;
Female;
Gene Frequency;
Genetics, Population;
Genome, Human;
Genotype;
Humans;
INDEL Mutation;
Male;
Multiplex Polymerase Chain Reaction/methods*;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2014;30(2):101-109
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate Insertion/Deletion (InDel) polymorphism on the X chromosome and to screen 18 InDel loci for the Chinese Han population as a forensic DNA typing system auxiliary.
METHODS:Eighteen X-InDel markers were selected using the Human Genome Browser and dbSNP database. Multiplex PCR primer pairs of selected X-InDel markers were designed using Primer 3 software and divided into 3 groups according to the amplified fragment length, labeled by FAM, HEX and TAMRA fluorescence dye, respectively. The population genetics research and comparative analysis of Chinese Han nationality and 4 main minorities, the Hui, Wei, Mongol, and Tibetan nationalities, were investigated with the system.
RESULTS:A new multiplex genotyping system, named InDel X-18PLEX, was successfully developed and validated, consisted of 18 X-InDel markers on the X chromosome and 1 Amelogenin gender marker. No deviation from Hardy-Weinberg equilibrium expectations was detected in the distribution of genotypes in the 5 investigated ethnic groups. However, there was significant difference between their distributions. From the investigation of Han nationality, high female (0.9999994) and male (0.999 88) overall discrimination power values were obtained, as well as high overall mean exclusion chance values in trios (0.999 992) and in duos (0.99).
CONCLUSION:InDel X-18PLEX meets the requirements as a forensic DNA complementary kit, providing effective supplementary analytical tools for difficult cases.
