Establishment of the multiplex genotyping system for 16 SNP loci on mtDNA.
- Author:
Dan WU
;
Yan-Chai NIE
;
Yu CAO
;
Yu CAO
;
Huai-Gu ZHOU
- Publication Type:Journal Article
- MeSH:
Alleles;
DNA;
DNA, Mitochondrial;
Genotype;
Genotyping Techniques;
Humans;
Polymerase Chain Reaction/methods*;
Polymorphism, Single Nucleotide;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2014;30(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a multiplex genotyping system of mtDNA SNP.
METHODS:A multiplex analysis system of 16-plex mtDNA SNP loci was established with allele specific PCR and capillary electrophoresis genotyping technology. Fifty samples from unrelated Chinese Han individuals were typed with the multiplex system. The multiplex assay was validated by comparing with the direct sequencing method.
RESULTS:The genotypes of all 50 samples were correctly determined by the multiplex system. The optimal genotypic graphs were obtained with an input DNA of 0.5-10 pg, and the typing results were completely consistent with those by direct sequencing method.
CONCLUSION:The established multiplex system by allele specific PCR has high sensitivity, operational simplicity and high accuracy. It provides an effective and high output method for mtDNA SNP typing.
