Expression of GAP-43 in midbrain ventral tegmental area of morphine withdrawal rats.
- Author:
Liang-ming LUO
1
;
Yu-feng WU
2
;
Wei-cheng NI
3
;
Hua ZHU
4
Author Information
1. Department of Forensic Medicine, Wenzhou Medical College, Wenzhou 325035, China. wzrsmart@gmail.com
2. Criminal Investigation Team, Zhuji Public Security Bureau, Zhuji 311800, China.
3. Department of Criminal Technology, Pingyang Public Security Bureau, Pingyang 325400, China.
4. Department of Forensic Medicine, Wenzhou Medical College, Wenzhou 325035, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Behavior, Animal/drug effects*;
Disease Models, Animal;
Female;
GAP-43 Protein/metabolism*;
Immunohistochemistry;
Male;
Mesencephalon/metabolism*;
Morphine/adverse effects*;
Morphine Dependence/metabolism*;
Naloxone/pharmacology*;
Rats;
Rats, Sprague-Dawley;
Substance Withdrawal Syndrome/metabolism*;
Time Factors;
Ventral Tegmental Area/metabolism*
- From:
Journal of Forensic Medicine
2013;29(5):330-336
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the protein expression of growth associated protein-43 (GAP-43) in midbrain ventral tegmental area in morphine withdrawal rats at different time, and to evaluate the effect of GAP-43 on morphine withdrawal memory.
METHODS:Rat models of morphine dependent 1 week, 2 weeks and 4 weeks were established by morphine hydrochloride intraperitoneal injection with increasing doses to establish natural withdrawal. The protein expression of GAP-43 in midbrain ventral tegmental area was observed by immunohistochemical staining and the results were analyzed by Image-Pro Plus 5.1 image analysis system.
RESULTS:With prolongation of dependent time, the expression of GAP-43 was decreased then increased in midbrain ventral tegmental area.
CONCLUSION:GAP-43 could play a role in morphine withdrawal memory in midbrain ventral tegmental area.
