Correlation between the changes of oxidation reduction potential values and postmortem interval of heart blood in rabbits after death.
- Author:
Tian-tong YANG
1
;
Yong-guang YU
2
;
Jing BAI
2
;
Hai-dong ZHANG
3
;
Ting-yi SUN
4
;
Mary G RIPPLE
5
;
David R FOWLER
5
;
Li LING
3
Author Information
1. China University of Political Science and Law, Key Laboratory of Evidence Science, Ministry of Education, Beijing 100040, China. yangtiantong@126.com
2. School of Science, Beijing Jiaotong University, Beiing 100044, China.
3. China University of Political Science and Law, Key Laboratory of Evidence Science, Ministry of Education, Beijing 100040, China.
4. Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
5. Office of the Chief Medical Examiner of Maryland, Baltimore 21223, USA.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Blood;
Female;
Forensic Pathology/methods*;
Heart;
Male;
Oxidation-Reduction;
Oxygen/analysis*;
Postmortem Changes;
Rabbits;
Regression Analysis;
Temperature;
Time Factors
- From:
Journal of Forensic Medicine
2013;29(5):321-329
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate correlation between the changes of oxidation reduction potential (ORP) values of heart blood in rabbits after death and postmortem interval (PMI) at different temperatures.
METHODS:Forty-eight rabbits were randomly divided into 6 groups and sacrificed by air embolism. Blood samples were taken from the right ventricle of each rabbit and stored at different temperatures of 10, 15, 20, 25, 30 and 35 degrees C, respectively. Every 4 hours from 0 h to 132 h postmortem, the ORP values of the blood samples were measured at different intervals by PB-21 electrochemical analyzer. The curvilinear regression equation was established by SPSS 17.0 software. The surface equation and 3D surface diagram were established by MATLAB 7.10.0 software.
RESULTS:The ORP values at different temperatures of heart blood in rabbits were highly correlated with the PMI. The ORP values rised obviously when the temperature was high and rised slowly when the temperature was low. The surface equation and 3D surface diagram were obtained.
CONCLUSION:The surface equation and 3D surface diagram of ORP values and PMI may be used for PMI estimation at different temperatures.
