Determination of the biological attribute of evidence at the scene using reverse transcription PCR and real-time fluorescent quantitative PCR.
- Author:
Yan XU
1
;
Chen ZHANG
2
;
Qing-Wen XU
2
;
Jiang-Ping HUANG
2
;
Ya-Nan LIU
2
;
Kai-Nan ZOU
2
;
Yuan PING
2
;
Huai-Gu ZHOU
2
Author Information
1. Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China. xxnnxy@hotmail.com
2. Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Blood Stains;
DNA/isolation & purification*;
DNA Primers;
Forensic Medicine/methods*;
Genetic Markers;
Humans;
Male;
Polymerase Chain Reaction/methods*;
RNA/isolation & purification*;
RNA, Messenger/analysis*;
Saliva;
Semen;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2013;29(4):259-262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the feasibility of biological method to identify the biological attribute of samples at crime scene.
METHODS:Thirty samples of ten blood stains, ten saliva stains and ten semen stains were selected, and all the samples were processed by the routine method and biomolecular method, respectively. Both RNA and DNA were isolated using DNA-RNA co-extraction technology and the mRNA was converted into cDNA using reverse transcription PCR (RT-PCR). Three pairs of specific primers were designed for blood stain, saliva stain and semen stain based on the different target genes in three specific tissues and the primers were amplified using real-time fluorescent quantitative PCR. The differences in these biological samples were evaluated by melting temperature (Tm) values and the size of the amplification fragment.
RESULTS:The Tm values of blood stain, saliva stain and semen stain were (84.5 +/- 0.2) degrees C, (76.9 +/- 0.3) degrees C and (88.5 +/- 0.2) degrees C, respectively. The length of PCR fragments of them was 177bp, 134bp and 294bp, respectively.
CONCLUSION:Compared with the routine method, RT-PCR with real-time fluorescent quantitative PCR is highly specific, sensitive and reliable to identify the biological attribute of evidence, and can be potentially applied to determine evidence attribute in forensic practice.
