Modified TRIzol method for RNA and DNA co-extraction from blood.
- Author:
Juan-Juan QIN
1
;
Zhi-Yong LU
;
Zhang-Ping JIAO
;
Xiao-Jun ZHU
;
Ying-Xi WANG
;
Hui TANG
Author Information
1. Department of Forensic Medicine, School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Blood Chemical Analysis/methods*;
DNA/isolation & purification*;
DNA Fingerprinting;
Forensic Medicine;
Guanidines/chemistry*;
Humans;
Hydrogen-Ion Concentration;
Phenols/chemistry*;
Polymerase Chain Reaction;
RNA/isolation & purification*;
Reagent Kits, Diagnostic
- From:
Journal of Forensic Medicine
2013;29(3):209-211
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a new method for RNA and DNA co-extraction from the same sample by TRIzol reagent.
METHODS:After the aqueous phase which contained total RNA was removed by traditional TRIzol method, the values of pH of the interphase phase and organic phase were adjusted. The DNA was precipitated with ethanol and purified with DNA IQ system. The purified DNA was measured in quality and quantity. As the template, it was amplified and typed by PCR-STR. The data was compared with that extracted by traditional TRIzol method.
RESULTS:The DNA extracted by this modified method showed a better result of quality and quantity than that by traditional TRIzol method and a good STR typing.
CONCLUSION:The modified TRIzol method is advisable and reliable to simultaneously extract both DNA and RNA from the same sample. It could be used for individual identification and paternity testing to satisfy the need of forensic science.
