Genetic polymorphisms of 15 X-STR loci in Shandong Han population.
- Author:
Teng MA
1
;
Jing XU
;
Ya-Jun YANG
;
Kuan SUN
;
Fu-Zhong XUE
;
Li JIN
;
Shi-Lin LI
Author Information
1. Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200433, China. mt152630@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Asian People/genetics*;
China;
Chromosomes, Human, X/genetics*;
DNA Fingerprinting;
DNA Primers;
Female;
Forensic Genetics;
Gene Frequency;
Genetic Linkage;
Genetics, Population;
Genotype;
Humans;
Male;
Microsatellite Repeats;
Multiplex Polymerase Chain Reaction;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2013;29(3):202-208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han population in order to establish the forensic application database.
METHODS:The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins (FAM, VIC, NED and TET). The developed multi-PCR was used to investigate 15 X-STR loci (DXS10011, DXS101, GATA 165B12, DXS6795, DXS6800, DXS6801, DXS6803, DXS7132, DXS7133, DXS7423, DXS7424, DXS8377, DXS8378, DXS9898 and HPRTB) selected from the X chromosome of 481 unrelated individuals (295 females and 186 males) in Shandong Han population.
RESULTS:Among the 15 X-STR loci, GATA 165B12, DXS6800, DXS6803, DXS7133 and DXS7423 showed moderate polymorphisms, while the rest 10 X-STR loci showed high polymorphisms (PIC > 0.5 and H > 0.5). No shared haplotype was detected among the males in Shandong Han population.
CONCLUSION:The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Han population and shows its forensic application.
