Myocardial expression of Spry1 and MAPK proteins of viral myocarditis.
- Author:
Hong-Fei XU
1
;
Lin MENG
;
Jian YAO
;
Zhen-Yong GU
;
Guo-Qing LIU
;
Yi-Wen SHEN
;
Zi-Qin ZHAO
Author Information
1. Department of Forensic Medicine, School of Biology and Basic Medical Sciences, Medical College of Soochow University, Suzhou 215123, China. whhongfei@sina.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Adaptor Proteins, Signal Transducing/metabolism*;
Animals;
Coxsackievirus Infections/pathology*;
Death, Sudden, Cardiac/pathology*;
Disease Models, Animal;
Immunohistochemistry;
Male;
Membrane Proteins/metabolism*;
Mice;
Mice, Inbred BALB C;
Mitogen-Activated Protein Kinases/metabolism*;
Myocarditis/virology*;
Myocardium/pathology*;
Phosphoproteins/metabolism*;
RNA, Messenger/metabolism*;
Random Allocation;
Real-Time Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2013;29(3):164-167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To discuss the myocardial expression of Spry1 and MAPK proteins of viral myocarditis (VMC), to reveal its mechanism of sudden death, and to provide guides for forensic identification of sudden cardiac death.
METHODS:Thirty Balb/c male mice were randomly divided into VMC group and control group, inoculated intraperitoneally with Coxsackievirus B3 and Eagel's solution, respectively. After the mice were sacrificed, the cardiac tissues of the mice were taken to proceed regular pathological examination. The changes of Spry1 protein, Spry1 mRNA and MAPK protein were detected by immunohistochemistry, Western blotting and real-time PCR.
RESULTS:Under light microscope, the pathologic changes included myocardial interstitial edema, inflammatory cells infiltration, myocardial necrosis, and focal and patchy necrosis of myocardial fiber in VMC group. The expression of Spry1 protein in VMC group was lower than that in control group (P < 0.05). There was slightly decreased expression of Spry1 of the mRNA level in VMC group (P > 0.05). But the MAPK protein expression in VMC group was higher than that in control group (P < 0.05).
CONCLUSION:The pathway of MAPK/ERK involving Spry1 protein accelerates the expression of collagen, which may contribute to arrhythmia, heart failure and even sudden cardiac death.
