DNA extraction from bones and teeth using AutoMate Express forensic DNA extraction system.
- Author:
Lin-Lin GAO
1
;
Nian-Lai XU
;
Wei XIE
;
Shao-Cheng DING
;
Dong-Jing WANG
;
Li-Qin MA
;
You-Ying LI
Author Information
1. Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310004, China. myhappyfamily_gao@163.com
- Publication Type:Journal Article
- MeSH:
Automation;
Bone and Bones/chemistry*;
DNA/isolation & purification*;
DNA Fingerprinting/methods*;
Forensic Medicine/methods*;
Humans;
Microsatellite Repeats;
Polymerase Chain Reaction;
Specimen Handling/methods*;
Tooth/chemistry*
- From:
Journal of Forensic Medicine
2013;29(2):127-129
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore a new method in order to extract DNA from bones and teeth automatically.
METHODS:Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate Express forensic DNA extraction system.
RESULTS:DNA extraction from bones and teeth were completed in 3 hours using the AutoMate Express forensic DNA extraction system. There was no statistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method.
CONCLUSION:AutoMate Express forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.
