Method development and validation for determining 1,3-butadiene in human blood by gas chromatography-mass spectrometry and head-space gas chromatography.
- Author:
Su-Jing ZHANG
1
;
Bao-Hua SHEN
;
Xian-Yi ZHUO
Author Information
1. College of Pharmaceutical Science, Soochow University, Suzhou 215123, China. ivy-yuonly@163.com
- Publication Type:Journal Article
- MeSH:
Adult;
Butadienes/poisoning*;
Forensic Toxicology/methods*;
Gas Chromatography-Mass Spectrometry/methods*;
Gas Poisoning;
Humans;
Male;
Reproducibility of Results;
Sensitivity and Specificity;
Solvents/chemistry*;
Temperature
- From:
Journal of Forensic Medicine
2013;29(2):110-115
- CountryChina
- Language:English
-
Abstract:
To develop a simple, validated method for identifying and quantifying 1,3-butadiene (BD) in human blood by gas chromatography-mass spectrometry (GC-MS) and head-space gas chromatography (HS-GC). BD was identified by GC-MS and HS-GC, and quantified by HS-GC. The method showed that BD had a good linearity from 50 to 500 microg/mL (r > 0.99). The limits of detection and quantification were 10 microg/mL and 50 microg/mL, respectively. Both the intra-day precision and inter-day precision were < 6.08%, and the accuracy was 96.98%-103.81%. The method was applied to an actual case, and the concentration of BD in the case was 242 microg/mL in human blood. This simple method is found to be useful for the routine forensic analysis of acute exposure to BD.
