Distribution of deltamethrin in acute poisoned rats.
- Author:
Bin WU
1
;
Peng YAN
;
Zhi-Wen WEI
;
Yu-Jin WANG
Author Information
1. College of Forensic Medicine, Shanxi Medical University, Taiyuan, China. luoyejingyou@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Chromatography, Gas/methods*;
Disease Models, Animal;
Forensic Toxicology/methods*;
Kidney/metabolism*;
Linear Models;
Liver/metabolism*;
Lung/metabolism*;
Male;
Nitriles/poisoning*;
Pyrethrins/poisoning*;
Rats;
Reproducibility of Results;
Sensitivity and Specificity;
Tissue Distribution
- From:
Journal of Forensic Medicine
2013;29(1):25-30
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an animal model in acute poisoned rat by deltamethrin and an analysis method for determination of deltamethrin by gas chromatography-electron capture detector (GC-ECD) and to study the distribution of deltamethrin in rats in order to provide the references for forensic medicine identification about such cases.
METHODS:Rats were administered with deltamethrin of different doses(512 and 1,024 mg/kg) and killed 1.5 h later to be dissected rapidly for tissues (blood, hearts, livers, lungs, kidneys and brains etc.). Samples were dehydrated by anhydrous sodium sulfate and extracted with petroleum ether and acetone (V:V=4:1). The level of deltamethrin was determined by GC-ECD.
RESULTS:There was a good separate between deltamethrin and endogenous impurities. The limit of quantification for deltamethrin in blood and liver were 0.1 microg/mL and 0.1 microg/g (S/N> or =10), respectively. The recovery rate of deltamethrin in blood was 91.55%-134.37% and both inter-day and intra-day precisions were less than 5.67%. The distribution of deltamethrin in poisoned rats with 512 mg/kg was as follow: lungs > livers > hearts > kidneys > blood > brains and with 1 024 mg/kg dose was lungs > blood > hearts > kidneys > brains > livers (P<0.05).
CONCLUSION:The GC-ECD method is sensitive for determination of deltamethrin. The distribution of deltamethrin in rats has a dose-dependent manner. The study suggests that samples of blood, hearts, livers, lungs, kidneys and brains are suitable for deltamethrin poisoned analysis.
