Forensic application of expressmarker 22 STR loci direct PCR amplification kit.
- Author:
Kai-Nan ZOU
1
;
Yu CAO
;
Zi-Fang XIA
;
Wei-Guo ZHENG
;
Huai-Gu ZHOU
Author Information
1. Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China. kitty_zkn@126.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
DNA/genetics*;
DNA Fingerprinting/methods*;
DNA Primers;
Forensic Genetics/methods*;
Genetic Loci/genetics*;
Genotype;
Humans;
Microsatellite Repeats;
Polymerase Chain Reaction/methods*
- From:
Journal of Forensic Medicine
2012;28(6):448-450
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit.
METHODS:One thousand nine hundred and forty-eight samples (including samples spotted on FTA cards, filter papers and case samples) were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using Sinofiler kit, Identifiler kit and PowerPlex 16 kit respectively for comparison. The genotypes were compared at the same STR loci among these four kits to test the sensitivity and accuracy of Expressmarker 22 STR loci direct PCR amplification kit.
RESULTS:97.79% samples were successfully typed using Expressmarker 22 STR loci direct PCR amplification kit. The genotype profiles of the same samples using Expressmarker 22 STR loci direct PCR amplification kit were consistent with Sinofiler kit, Identifiler kit and PowerPlex 16 kit at the same STR loci.
CONCLUSION:Expressmarker 22 STR loci direct PCR amplification kit can provide huge information and accurate results
