Genetic polymorphisms of the dinucleotide STR locus D6S261.
- Author:
Peng YANG
1
;
Ya-Nan LIU
;
Yan-Chai NIE
;
Huai-Gu ZHOU
;
Zi-Qin ZHAO
Author Information
1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Asian People/genetics*;
Base Sequence;
Forensic Genetics/methods*;
Gene Frequency;
Genotype;
Humans;
Microsatellite Repeats/genetics*;
Nucleotides/genetics*;
Paternity;
Polymerase Chain Reaction/methods*;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2012;28(6):445-447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the application of dinucleotide STR locus in paternity testing.
METHODS:Dinucleotide STR locus D6S261 was selected and the paternity testing blood samples were amplified using 200 random blood samples, 16 family samples and 193 paternity test samples. Data of the PCR products were collected by 3130XL Genetic Analyzer and the genetic parameters of population were calculated by PowerStats v12.
RESULTS:Fifteen alleles and 50 genotypes were found and H, DP, PE and PIC were 0.850, 0.953, 0.695, and 0.820, respectively. The typing results of both family samples and paternity test samples were accord with the law of inheritance, which no mutation was discovered.
CONCLUSION:The genetic polymorphisms of D6S261 show good characteristics with low mutation rate and high stability. It can be an effective method to solve the indetermination caused by mutation in paternity testing if the stutter bands can be decreased.
